Single-Molecule Angular Optical Trapping for Studying Transcription Under Torsion.
J. Ma, C. Tan, and M.D. Wang
Methods Mol Biol. (2018) (Springer).

Transcription Factor Regulation of RNA polymerase’s Torsional Capacity
J. Ma, C. Tan, X. Gao, R. Fulbright, J. Roberts, and M.D. Wang
bioRxiv. (2018) (bioRxiv).

Helicase promotes replication re-initiation from an RNA transcript
B. Sun, A. Singh, S. Sultana, J.T. Inman, S.S. Patel, and M.D. Wang
Nature Communications. 9:2306. (2018) (Nature Communications).

Mfd Dynamically Regulates Transcription via a Release and Catch-up Mechanism
T.T. Le, Y. Yang, C. Tan, M.M. Suhanovsky, R.M. Fulbright, J.T. Inman, M. Li, J. Lee, S. Perelman, J.W. Roberts, A.M. Deaconescu, and M.D. Wang
Cell. 172:344-357 (2018) (Cell).

Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome
B. Sun, and M.D. Wang
Methods in Enzymology. Volume 582, 55-84. (2017) (Full Text PDF).

Nanophotonic trapping: precise manipulation and measurement of biomolecular arrays
J.E. Baker, R.P. Badman, and M.D. Wang
WIREs Nanomed Nanobiotechnol e1477. (2017) (WIREs).

Tunable nanophotonic array traps with enhanced force and stability
F. Ye, M. Soltani, J.T. Inman, and M.D. Wang
Optics Express 25(7):7907-7918. (2017) (OSA Publishing).

DNA looping mediates nucleosome transfer
L.D. Brennan, R.A. Forties, S.S. Patel, and M.D. Wang
Nature Communications 7: 13337. (2016) (Full Text PDF).

Biocompatible and High Stiffness Nanophotonic Trap Array for Precise and Versatile Manipulation
F. Ye, R.P. Badman, J.T. Inman, M. Soltani, J.L. Killian, and M.D. Wang
Nano Letters 16(10), 6661-6667. (2016) (Full Text PDF).

The Chd1 chromatin remodeler can sense both entry and exit sides of the nucleosome
I.M. Nodelman, K.C. Horvath, R.F. Levendosky, J. Winger, R. Ren, A. Patel, M. Li, M.D. Wang, E. Roberts, and G.D. Bowman
NAR (2016) (Full Text PDF).

DNA supercoiling during transcription
J. Ma and M.D. Wang
Biophys. Rev. (2016) (Full Text PDF).

Single-molecule perspectives on helicase mechanisms and functions
B. Sun and M.D. Wang
Crit. Rev. Biochem. Mol. Biol. 51:1, 15-25 (2016) (Full Text PDF).

Dynamic regulation of transcription factors by nucleosome remodeling
M. Li, A. Hada, P. Sen, L. Olufemi, M. A. Hall, B. Y. Smith, S. Forth, J. N. McKnight, A. Patel, G. D. Bowman, B. Bartholomew, and M. D. Wang
eLIFE 4: e06249. (2015) (Full Text PDF).

T7 replisome directly overcomes DNA damage
B. Sun, M. Pandey, J. T. Inman, Y. Yang, M. Kashlev, S. S. Patel, and M. D. Wang
Nature Communications 6: 10260. (2015) (Full Text PDF).

Interplay between DNA supercoiling and transcription elongation
J. Ma and M.D. Wang
Transcription 5: e28636. (2014) (Full Text PDF).

DNA Y Structure: A Versatile, Multidimensional Single Molecule Assay
J. T. Inman, B. Y. Smith, M. A. Hall, R. A. Forties, J. Jin, J. P. Sethna, and M. D. Wang
Nano Letters 14.11: 6475-6480. (2014) (Full Text PDF).

RNA polymerase is a powerful torsional motor.
J. Ma and M.D. Wang
Cell Cycle 13.3: 337–338 (2014) (Full Text PDF).

Discovering the power of single molecules.
R.A. Forties and M.D. Wang
Cell 157.1: 4-7 (2014) (Full Text PDF).

Nanophotonic Trapping for Precise Manipulation of Biomolecular Arrays.
M. Soltani, J. Lin, R.A. Forties, J.T. Inman, S.N. Saraf, R.M. Fulbright, M. Lipson, and M.D. Wang
Nature Nanotechnology 9.6: 448-452 (2014) (Full Text PDF).

Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.
Y. Wang, S. Maharana, M.D. Wang, and G.V. Shivashankar
Science Reports 4 (2014) (Full Text PDF).

On the move.
R.A. Forties, J. Ma, and M.D. Wang
eLife 10.7554/eLife.01414 (2013) (Full Text PDF).

Torque modulates nucleosome stability and facilitates H2A/H2B dimer loss.
M.Y. Sheinin, M. Li, M. Soltani, K. Luger, and M.D. Wang
Nature Communications 4:2579-86 (2013) (Full Text PDF).

Small molecule injection into single-cell C. elegans embryos via carbon-reinforced nanopipettes.
L.D. Brennan, T. Roland, D.G. Morton, S.M. Fellman, S.Y. Chung, M. Soltani, J.W. Kevek, P.M. McEuen, K.J. Kemphues, and M.D. Wang.
PlosONE 8(9) (2013) (Full Text PDF).

Single-molecule unzipping force analysis of HU-DNA complexes.
R.T. Dame, M.A. Hall and M.D. Wang
ChemBiochem 14:1954-57 (2013) (Full Text PDF).

Transcription under torsion.
J. Ma, L. Bai, and M.D. Wang
Science 340:1580-3 (2013) (Full Text PDF).

Torque measurement at the single-molecule level.
S. Forth, M.Y. Sheinin, J. Inman, and M.D. Wang
Annu. Rev. Biophys. (2013) (Full Text PDF).

DNA twist diffuses and hops.
M.Y. Sheinin and M.D. Wang.
Science 38:56-7 (2012) (Full Text PDF).

Electro-optofluidics: achieving dynamic control on-chip.
M. Soltani, J.T. Inman, M. Lipson, and M.D. Wang
Opt. Express (2012) (Full Text PDF).

Unzipping single DNA molecules to study nucleosome structure and dynamics.
M. Li and M.D. Wang
Methods Enzymol. (2012) (Full Text PDF).

Recent Advances in Single Molecule Studies of Nucleosomes.
J.L. Killian, M. Li, M.Y. Sheinin, and M.D. Wang
Curr. Opin. Struc. Biol. (2012) (Full Text PDF).

ATP-induced helicase slippage reveals highly coordinated subunits.
B. Sun, D.S. Johnson, G. Patel, B.Y. Smith, M. Pandey, S.S. Patel, and M.D. Wang
Nature 478:132 (2011) (Full Text PDF).

Underwound DNA under tension: structure, elasticity, and sequence-dependent behaviors.
M.Y. Sheinin, S. Forth, J.F. Marko, and M.D. Wang.
Phys. Rev. Lett. 107: 108102 (2011) (Full Text PDF).

Direct Measurements of Torque During Holliday Junction Migration.
S. Forth, C. Deufel., S.S Patel, and M.D. Wang.
Biophys. J. 101: L05-L07 (2011) (Full Text PDF).

The A257T linker region mutant of T7 helicase-primase protein is defective in DNA binding and rescued by T7 DNA polymerase.
G. Patel, D.S. Johnson, B. Sun, M. Pandey, X. Yu, E.H. Egelman, M.D. Wang, and S.S. Patel.
J. Biol. Chem. 286: 20490-9 (2011) (Full Text PDF).

Angular optical trapping.
S. Forth and M.D. Wang.
In Encyclopedia of Biophysics, edited by N.G. Walter (2011).

Structure and Scm3-mediated assembly of budding yeast centromeric nucleosomes.
M.L. Dechassa, K. Wyns, M. Li, M.A. Hall; M.D. Wang, K. Luger.
Nat. Commun. 2:313 (2011) (Full Text PDF).

Comparison of pause predictions of two sequence-dependent transcription models.
Lu Bai and Michelle D Wang
Journal of Statistical Mechanics: Theory and Experiment (2010) (Full Text PDF).

Passive torque wrench and angular position detection using a single-beam optical trap.
J. Inman, S. Forth, and M. D. Wang
Optics Letters, Vol. 35, Issue 17, pp. 2949-2951 (2010) (Full Text PDF).

Synergistic action of RNA polymerases in overcoming the nucleosomal barrier.
J. Jin, L. Bai, D.S. Johnson, R.M. Fulbright, M.L. Kireeva, M. Kashlev, and M.D. Wang
Nature Structural and Molecular Biology (2010) (Full Text PDF).

Discontinuities at the DNA supercoiling transition.
B. Daniels, S. Forth, M.Y. Sheinin, M.D. Wang, and J.P. Sethna.
Physical Review E 80:040901(R) (2009) (Full Text PDF).

Twist-stretch coupling and phase transition during DNA supercoiling.
M.Y. Sheinin and M.D. Wang
Phys. Chem. Chem. Phys. 11:4800-4803 (2009) (Full Text PDF).

High-resolution dynamic mapping of histone-DNA interactions in a nucleosome
M.A. Hall, A. Shundrovsky, L. Bai, R.M. Fulbright, J.T. Lis, and M.D. Wang.
Nature Structural and Molecular Biology 16:124 (2009) (Full Text PDF).

Kinetic modeling of transcription elongation.
L. Bai, A. Shundrovsky, and M.D. Wang.
In “RNA polymerases as molecular motors” by the Royal Society of Chemistry (2009) (Full Text PDF).

Abrupt Buckling Transition Observed during the Plectoneme Formation of Individual DNA Molecules.
S. Forth, C. Deufel, M.Y. Sheinin, B. Daniels, J.P. Sethna, and M.D. Wang.
Physical Review Letters 100:148301 (2008) (Full Text PDF).

Single molecule studies of RNA polymerase transcription.
A. Shundrovsky, L. Bai, T.J. Santangelo, and M.D. Wang.
In “Escherichia coli and Salmonella typhimurium EcoSal: Cellular and Molecular Biology”, eds. I. Artsitmovitch (2008).

A biophysicist marvels at the idea of grabbing microscopic particles with light by tweaking its phase.
M.D. Wang.
Nature 454:921 (2008).

Single molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase.
D.S. Johnson, L. Bai, B.Y. Smith, S.S. Patel, and M.D. Wang.
Cell 129:1299-1309 (2007) (Full Text PDF).

Nanofabricated quartz cylinders for angular optical trapping: torque detection during DNA supercoiling.
C. Deufel, S. Forth, C.R. Simmons, S. Dejgosha, and M.D. Wang.
Nature Methods 4:223-5 (2007) (Full Text PDF).

Mechano-chemical kinetics of transcription elongation.
L. Bai, R.M. Fulbright, & M.D. Wang.
Physical Review Letters 98:068103 (2007) (Full Text PDF).

Probing SWI/SNF remodeling of the nucleosome by unzipping single DNA molecules.
A. Shundrovsky, C. L. Smith, J.T. Lis, C.L. Peterson, and M.D. Wang.
Nature Structural and Molecular Biology 13:549-54 (2006) (Full Text PDF).

Single-molecule analysis of RNA polymerase transcription.
L. Bai, T.J. Santangelo, and M.D. Wang.
Annual Review of Biophysics and Biomolecular Structure 35:343-60 (2006) (Full Text PDF).

Detection of forces and displacements along the axial direction in an optical trap.
C. Deufel and M.D. Wang.
Biophysical Journal 90:657-67 (2006) (Full Text PDF).

Detection of high affinity mismatch binding and sliding clamp modes for the MSH2-MSH6 mismatch recognition complex by single-molecule unzipping force analysis.
J. Jiang, L. Bai, J. Surtees, Z. Gemici, M.D. Wang, and E. Alani.
Molecular Cell 20:771-81 (2005) (Full Text PDF).

Functional hydrogel surfaces: binding kinesin-based molecular motor proteins to selected patterned sites.
T. Yu, Q. Wang, D.S. Johnson, M.D. Wang, and C.K. Ober.
Advanced Functional Materials 15: 1303-1309 (2005) (Full Text PDF).

Specific contributions of histone tails and their acetylation to the mechanical stability of nucleosomes.
B. Brower-Toland, D.A. Wacker, R.M. Fulbright, J.T. Lis, W.L. Kraus, and M.D. Wang.
Journal of Molecular Biology 346:135-146 (2005) (Full Text PDF).

A single molecule technique to study sequence-dependent transcription pausing.
A. Shundrovsky, T.J. Santangelo, J.W. Roberts, and M.D. Wang.
Biophysical Journal 87:3945-53 (2004) (Full Text PDF).

Sequence-dependent kinetic model for RNA polymerase elongation.
L. Bai, A. Shundrovsky, and M.D. Wang.
Journal of Molecular Biology 344:335-349 (2004) (Full Text PDF).

The molecular mechanism of transcription inhibition by Microcin J25.
K. Adelman, J. Yuzenkova, A. La Porta, J.T. Lis, S. Brukhov, M.D. Wang, and K.V. Severinov.
Molecular Cell 14:753-762 (2004) (Full Text PDF).

Optical torque wrench: angular trapping, rotation and torque detection using quartz microparticles.
A. La Porta and M.D. Wang.
Physical Review Letters 92:190801 (2004) (Full Text PDF).

Use of optical trapping techniques to study single nucleosome dynamics.
B. Brower-Toland, and M.D. Wang.
Methods in Enzymology: Chromatin and Chromatin Remodeling Enzymes 376:62-72 (2004). (Full Text PDF).

Dynamic force spectroscopy of protein-DNA interactions by unzipping DNA double helix.
S.J. Koch, and M.D. Wang.
Physical Review Letters 91:028103 (2003) (Full Text PDF).

Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior.
K. Adelman, A. La Porta, T.J. Santangelo, J.T. Lis, J.W. Roberts, and M.D. Wang.
Proceedings of the National Academy of Sciences, USA 99:13538 (2002) (Full TextPDF).

Probing protein-DNA interactions by unzipping a single DNA double helix.
S.J. Koch, A. Shundrovsky, B.C. Jantzen, M.D. Wang.
Biophysical Journal 83:1098 (2002) (Full Text PDF).

Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA.
B. Brower-Toland, R.C. Yeh, C. Smith, C.L. Peterson, J.T. Lis, and M.D. Wang.
Proceedings of the National Academy of Sciences, USA 99:1960 (2002) (Full TextPDF).

  • New insights into unwrapping DNA from the nucleosome from a single-molecule optical tweezers method.
    Commentary by Jeffrey J. Hayes and Jeffrey C. Hansen. ibid 99:1752 (2002) (Full Text PDF).

Stretching of single collapsed DNA molecules.
C.G. Baumann, V.A. Bloomfield, S.B. Smith, C. Bustamante, M.D. Wang, and S.M. Block.
Biophysical Journal 78:1965-78 (2000) (Full Text PDF).

Manipulation of single molecules in biology.
Wang, M.D.
Current Opinion In Biotechnology 10:81-86 (1999) (Full Text PDF).

Estimating the persistence length of a worm-like-chain molecule from force-extension measurements.
C. Bouchiat, M.D. Wang, J.F. Allemand, T. Strick, S.M. Block, and V. Croquette.
Biophysical Journal 76:409-413 (1999) (Full Text PDF).

Force and velocity measured for single molecules of RNA polymerase.
M.D. Wang, M.J. Schnitzer, H. Yin, R. Landick, J. Gelles, and S.M. Block.
Science 282:902-907 (1998) (Research Article) (Full Text PDF).

Stretching DNA with optical tweezers.
M.D. Wang, H. Yin, R. Landick, J. Gelles, and S.M. Block.
Biophysical Journal 72:1335-1346 (1997). (Full Text PDF).

Transcription against an applied force.
H. Yin, M.D. Wang, K. Svoboda, R. Landick, J. Gelles, and S.M. Block.
Science 270:1653-1657 (1995) (Full Text PDF).

  • RNA polymerase gets very pushy.
    News by C. O’Brein.
    ibid 270:1568 (1995) (Full Text PDF).