We study the mechanisms, kinetics, and regulation of DNA transcription with an emphasis on the effects of molecular-scale forces and torques.
We study the molecular motors that carry out DNA replication.
The basis of gene regulation is maintained by the structure and organization of chromatin. We investigate chromatin structure, nucleosome-DNA interactions and dynamics, and the molecular motors that alter them.
We are beginning to probe compelling questions in developmental biology such as the establishment of cell polarity in
Techniques and Instrumentation
Angular Optical Trapping
The Wang lab built the world’s first angular optical trap, enabling us to simultaneously measure and control torque in biological systems. We are illuminating the important role torque plays in regulating the actions of proteins that translocate along DNA’s helical groove.
DNA unzipping is a technique in which the two strands of a single molecule of double-stranded DNA are mechanically separated into two single strands. The Wang lab was the first to develop this technique as a tool to probe the locations and interaction strengths of DNA-bound proteins.
The Wang lab is using nanophotonic techniques to develop on-chip optical trapping devices with extremely high stability and high throughput. These devices are intended to greatly increase productivity and make optical trapping techniques available to a broader community of researchers.
We fabricate carbon nanopipettes with tip diameters of ~100 nm, enabling us to locally inject living cells with minimal disruption and damage.
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